Table 1 Bacterial strains and plasmids used in this study.

Strains

E. coli TOP10

F-mcrA Δ(mrr-hsdRMS-mcrBC)Φ80lacZΔM15 ΔlacX74 deoR recA1 araΔ139 Δ(ara-leu)7697

Invitrogen

S. aureus ATCC 13709

American Type Culture Collection

P. aeruginosa PAO1

American Type Culture Collection

B. pseudomallei

1026b

Clinical isolate; Km^r Gm^r Sm^r Pm^r Tc^s Tp^s

[16]

DD503

1026b derivative; allelic exchange strain; Δ(amrR-oprA) (Km^s Gm^s Sm^s) rpsL (Sm^r)

[17]

SZ210

DD503 derivative; ΔwcbB

[18]

SR5

wcbB::Tn5OT182 Tcr

[14]

MB301

DD503 derivative; waaE::dhfrIIb-p15AoriV; Zeo^r

[19]

SRM117

1026b derivitive; wbiI::Tn5-OT182; Tc^r

[20]

RM330

DD503 derivative; phaC::pGSV3-lux

This study

RM0721

DD503 derivative; BPSL0721::pGSV3-lux

This study

B. mallei

ATCC 23344

Type strain genome sequence complete

American Type Culture Collection

Plasmids pGSV3-lux

Mobilizable suicide vector containing lux operon from Photorhabdus luminescens; OriT; Gm^r

[12]

pCR2.1-TOPO

Topoisomerase-mediated cloning vector; Ap^r Km^r

Invitrogen