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Table 1 Bacterial strains and plasmids used in this study.

From: Survival of Burkholderia pseudomallei in Water

Strain or plasmid Description Reference or source
E. coli TOP10 F-mcrA Δ(mrr-hsdRMS-mcrBC)Φ80lacZΔM15 ΔlacX74 deoR recA1 araΔ139 Δ(ara-leu)7697 Invitrogen
S. aureus ATCC 13709   American Type Culture Collection
P. aeruginosa PAO1   American Type Culture Collection
B. pseudomallei   
1026b Clinical isolate; Kmr Gmr Smr Pmr Tcs Tps [16]
DD503 1026b derivative; allelic exchange strain; Δ(amrR-oprA) (Kms Gms Sms) rpsL (Smr) [17]
SZ210 DD503 derivative; ΔwcbB [18]
SR5 wcbB::Tn5OT182 Tcr [14]
MB301 DD503 derivative; waaE::dhfrIIb-p15AoriV; Zeor [19]
SRM117 1026b derivitive; wbiI::Tn5-OT182; Tcr [20]
RM330 DD503 derivative; phaC::pGSV3-lux This study
RM0721 DD503 derivative; BPSL0721::pGSV3-lux This study
B. mallei   
ATCC 23344 Type strain genome sequence complete American Type Culture Collection
Plasmids pGSV3-lux Mobilizable suicide vector containing lux operon from Photorhabdus luminescens; OriT; Gmr [12]
pCR2.1-TOPO Topoisomerase-mediated cloning vector; Apr Kmr Invitrogen