Table 3 Number of validated genes of two serial dilutions of 3 cDNA samples before and after their preamplification.

Sample number

N° validated genes (CT ≤ 31)

N° validated genes with ΔΔCT outside ± 1.5

r

NPA samples

PA samples

2

39

46

4

0.978

11

41

46

3

0.956

22

40

45

1

0.968

ng cDNA/port

500

15000

Dilution 1/20 of the initial cDNA

1.25 ng of preamplified cDNA

Sample number

N° validated genes (CT ≤ 31)

N° validated genes with ΔΔCT outside ± 1.5

r

NPA samples

PA samples

2

14

38

6

0.972

11

22

40

2

0.967

22

6

39

5

0.988

ng cDNA/port

25

750

Dilution 1/400 of the initial cDNA

0.06 ng of preamplified cDNA

Sample number

N° validated genes (CT ≤ 31)

N° validated genes with ΔΔCT outside ± 1.5

r

NPA samples

PA samples

2

3

17

0

0.962

11

2

27

3

0.883

22

3

20

2

0.932

ng cDNA/port

1.25

37.5