Table 1 Oligonucleotides used for PCR
cDNA | Primer pair | no. of cycles |
|---|---|---|
β-actin | 5'-CCATCCTGCGTCTGGACCTG-3' 5'-GTAACAGTCCGCCTAGAAGC-3' | 25 |
β-casein | 5'-CCATCCTGCGTCTGGACCTG-3' 5'-GGAATGTTGTGGAGTGGCAG-3' | 25 |
WAP | 5'-AAAAGCCAGCCCCATTGAGG-3' 5'-AGGGTTATCACTGGCACTGG-3' | 30 |
AP-2α | 5'-CCACTCCTACTGCTGCTGCTACTCT-3' 5'-GTTCACAAACGCGACAGAACTTTTC-3' | 30 |
AP-2β | 5'-TAACATATTGGATTGGCTTTGGAGG-3' 5'-CTCACGTTTTATTTTCCAAAAGGGG-3' | 35 |
AP-2γ | 5'-ATCACACCTTCTGGTAGGAGGCAG-3' 5'-AGGCTTAGAGGTCCAGTCCCAATC-3' | 30 |
AP-2δ | 5'-AATCTATTTCCAGAGAGTCTTGCTGC-3' 5'-GATTTCATTTTATGGAGGGCTCAGG-3' | 30 |
AP-2ε | 5'-AAATGTGGGATTCTATTCAGGCCAG-3' 5'-ACAGGACTGTGGTAATGCAGCCTA-3' | 30 |