Analysis of gene expression patterns by microarray hybridization in blood mononuclear cells of SLA-DRB1 defined Canadian Yorkshire pigs

Table 2 Gene-specific primers and PCR conditions for relative quantification in the Light Cycler system

Gene name	GenBank^a	Primers (5'-> 3') ^b	Prod. size (bp) ^c	Ann. temp. (°C) ^d	Acq. temp. (°C) ^e
TLR2	AB085935.1	F TGCGAATCCTGAAAATAGGC	343	59	84
		R CTTGCGTCAGTGATTTCTGC
CCL4	NM_001075147	F GAAGCTCTGCGTGACTGTCC	391	59	87
		R AGGAACAGGATCTGCTGAGG
IL1B	NM_214055.1	F GCAGATGGTGTCTGTCATCG	444	60	84
		R TTCTCCATGTCCCTCTTTGG
SLA-DQA	AY191777.1	F TGTGGAGGTGAAGACATTGC	315	59	83
		R CAGCATCACTGGAGACTTGG
CASP1	NM_214162.1	F GAGAAAATCTCACCGCTTCG	572	59	83
		R AGTCACTCTTTCGGCAGTGG
RPL19	AV600389	F ATGAGACCAATGAAATCGCC	504	60	87
		R CATGAGGATCCGCTTGTTTT

^a Sequenced used for primer design; ^b Sequence of forward (F) and reverse (R) primers in 5' to 3' orientation; ^c Size of the amplified PCR product; ^d Annealing temperature and ^e acquisition temperature for qPCR.

ISSN: 1756-0500