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Figure 3 | BMC Research Notes

Figure 3

From: Biologically active fibroblast growth factor 1 tagged with various epitopes

Figure 3

HPLC analysis of the affinity of semipurified FGF1s for heparin. The elution profiles of FGF1, FGF1-myc-(His)6, FGF1-3xFLAG-(His)6 and FGF1-V5-(His)6 are illustrated in (a). NaCl concentration was gradually increased as illustrated at the top, and the absorbance at 280 nm was monitored. An aliquot of the peak fraction was also subjected for dot-blot analysis using anti-FGF1 monoclonal antibody (b).

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