Table 2 qRT-PCR assays used to evaluate potential reference genes in Atlantic cod. Gene short names, Genbank accession numbers, PCR primer sequences, amplicon sizes and PCR efficiencies (mean ± STDEV in six tissues) are shown.
Gene | Accession no. | Forward primer (5' – 3') | Reverse primer (5' – 3') | Amplicon size (bp) | PCR eff. |
|---|---|---|---|---|---|
ACTB | EX739174 | CACAGCCGAGCGTGAGATT | ACGAGCTAGAAGCGGTTTGC | 95 | 2.03 ± 0.09 |
ARP-2 | EX741634 | TCTGCTCCGTGTGGAAGTTG | CGAGAAGATCCTCTGCCACAA | 131 | 2.06 ± 0.04 |
EF1A | EX721840 | CCCTGTGGAAGTGGCTGAAG | CATCCAAGGGTCCGTATCTCTT | 93 | 2.02 ± 0.11 |
Ubi | EX735613 | GGCCGCAAAGATGCAGAT | CTGGGCTCGACCTCAAGAGT | 69 | 1.91 ± 0.09 |
GAPDH | EX725566 | CCATGACAACTTTGGCATCGT | AGGGTCCGTCCACTGTCTTCT | 83 | 2.16 ± 0.07 |
RPS9 | EX726043 | TCTTTGAAGGTAATGCTCTGTTGAGA | CGAGGATGTAATCCAACTTCATCTT | 84 | 1.94 ± 0.08 |
ARP | EX741373 | TGATCCTCCACGACGATGAG | CAGGGCCTTGGCGAAGA | 113 | 1.95 ± 0.10 |
RPL4 | EX725958 | GGTGCCATACAGCTGATCCA | CCAGGCATCACACTGCAGAA | 123 | 1.98 ± 0.12 |
RPL22 | EX727868 | GTTACCGGTCTTCCCGTTGA | AGAAGTCCAAAAAAGGAGCTTCCT | 132 | 1.79 ± 0.08 |
RPL37 | EX738140 | CCGAGAAGCGCAAGAGAAAG | GGTGGTACCTTCCCGGAATC | 134 | 1.87 ± 0.06 |