Table 3 Evaluation of the usefulness of six potential reference genes in eight tissues of Atlantic cod ranked by the Bestkeeper software. 1 (bold) = best, 10 = worst. Six individuals from four populations (one with n = 5, total n = 23) were analyzed for 10 genes in six tissues. Ranking of reference genes according to the Bestkeeper software in six tissues of Atlantic cod. n = 23.
Gene | Gills | Brain | Liver | Head kidney | Muscle | Intestine |
|---|---|---|---|---|---|---|
ACTB | 10 | 9 | 10 | 9 | 9 | 10 |
EF1A | 8 | 8 | 6 | 8 | 1 | 9 |
ARP-2 | 7 | 6 | 5 | 6 | 2 | 5 |
RPS9 | 4 | 3 | 4 | 3 | 7 | 6 |
ARP | 1 | 1 | 2 | 4 | 5 | 3 |
GAPDH | 9 | 10 | 9 | 10 | 10 | 8 |
Ubi | 3 | 2 | 1 | 2 | 3 | 2 |
RPL4 | 6 | 5 | 8 | 7 | 8 | 7 |
RPL22 | 5 | 7 | 3 | 5 | 4 | 4 |
RPL37 | 2 | 4 | 7 | 1 | 6 | 1 |