Figure 3From: The transcriptional cofactor MIER1-beta negatively regulates histone acetyltransferase activity of the CREB-binding proteinMIER1β does not interfere with H4 peptide binding by CBP. In vitro translated, 35S-labelled CBP1094–2441 was incubated with 0.1 ug biotinylated H4 peptide (Upstate Biotechnology Inc.) in the absence (lane 1) or presence of a 400-fold molar excess of GST (lane 2) or GST-MIER1β fusion protein (lane 3).Back to article page