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Table 2 PCR reaction parameters

From: Differential susceptibility of PCR reactions to inhibitors: an important and unrecognised phenomenon

     

Reaction parameters

Assay

F & R

[Primer]

[Probe]

Annealing

temp

95°C

Anneal

72°C

Wavelength

excite/acquire

Pj HSP70a

600 nM

200 nM

60°C

10 sec

10 sec

20 sec

530 nm/555 nm

mtLSU133

700 nM

100 nM

56°C

10 sec

20 sec

20 sec

530 nm/555 nm

CFP32

600 nM

75 nM

60°C

10 sec

20 sec

16 sec

530 nm/555 nm

IS1081

600 nM

75 nM

60°C

10 sec

20 sec

16 sec

530 nm/555 nm

16S MTb

600 nM

200 nM

60°C

10 sec

20 sec

20 sec

530 nm/555 nm

SPUD

600 nM

200 nM

56°C

10 sec

10 sec

20 sec

470 nm/510 nm