From: Differential susceptibility of PCR reactions to inhibitors: an important and unrecognised phenomenon
 |  |  |  |  | Reaction parameters | ||
---|---|---|---|---|---|---|---|
Assay | F & R [Primer] | [Probe] | Annealing temp | 95°C | Anneal | 72°C | Wavelength excite/acquire |
Pj HSP70a | 600 nM | 200 nM | 60°C | 10 sec | 10 sec | 20 sec | 530 nm/555 nm |
mtLSU133 | 700 nM | 100 nM | 56°C | 10 sec | 20 sec | 20 sec | 530 nm/555 nm |
CFP32 | 600 nM | 75 nM | 60°C | 10 sec | 20 sec | 16 sec | 530 nm/555 nm |
IS1081 | 600 nM | 75 nM | 60°C | 10 sec | 20 sec | 16 sec | 530 nm/555 nm |
16S MTb | 600 nM | 200 nM | 60°C | 10 sec | 20 sec | 20 sec | 530 nm/555 nm |
SPUD | 600 nM | 200 nM | 56°C | 10 sec | 10 sec | 20 sec | 470 nm/510 nm |