Table 3 Primer and amplicon characteristics
From: Differential susceptibility of PCR reactions to inhibitors: an important and unrecognised phenomenon
| Â | Primer | Amplicon | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
Assay | F/R | Length bp | Mw | Tm°C | %GC | dG free energy | 3' end stability | dH enthalpy of the oligo | Ds entropy of the oligo | 5' end dG | Size bp | % GC |
Pj HSP70a | F | 23 | 6949 | 59.43 | 43.48 | -37.01 | -8.51 | -170.1 | -0.45 | -8.13 | 108 | 47.2 |
| Â | R | 20 | 6053 | 57.87 | 55 | -34.14 | -6.47 | -153.4 | -0.4 | -7.58 | Â | Â |
mtLSU133 | F | 20 | 6166 | 54.91 | 50 | -32.16 | -9.31 | -146.8 | -0.38 | -8.03 | 133 | 32.2 |
| Â | R | 19 | 5777 | 54.46 | 52.63 | -31.51 | -8.53 | -142.9 | -0.37 | -6.24 | Â | Â |
CFP32 | F | 20 | 6233 | 57.32 | 50 | -34.59 | -8.57 | -158.5 | -0.42 | -6.72 | 63 | 58.7 |
| Â | R | 18 | 5547 | 55.49 | 61.11 | -30.83 | -7.58 | -134.5 | -0.35 | -9.6 | Â | Â |
IS1081 | F | 22 | 6638 | 69.72 | 63.64 | -41.97 | -13.43 | -174.5 | -0.44 | -8.29 | 135 | 67.4 |
| Â | R | 21 | 6496 | 67.86 | 61.9 | -40.00 | -8.48 | -166.8 | -0.43 | -9.5 | Â | Â |
16S MTb | F | 20 | 6175 | 56.81 | 50 | -33.71 | -7.58 | -153.3 | -0.4 | -6.84 | 71 | 54.9 |
| Â | R | 20 | 6062 | 55.73 | 55 | -32.04 | -5.59 | -143.4 | -0.37 | -8.27 | Â | Â |
SPUD | F | 23 | 6999 | 62.07 | 43.48 | -39.56 | -8.2 | -180.2 | -0.47 | -6.83 | 101 | 42.6 |
| Â | R | 23 | 6928 | 61.77 | 43.48 | -37.62 | -8.32 | -167.4 | -0.44 | -6.71 | Â | Â |