Table 1 Confirmation primers designed and directly re-amplified from HLB-infected samples.
Target region | Forward primers (5'-3') | Reverse Primers (5'-3') | Amplicon size (bp) |
|---|---|---|---|
Region-1 | |||
Region-1-L-F GGTCAATTCAATGCGCTATAC | Region-1-L-R CAGGAATCTGTCGAGCAATGG | 3,308 | |
Region-1-R-F1 GGCATGTGTTGGTCTTGGAA | Region-1-R-R1 TCGGTGATTCAATAATTTCC | 3,019 | |
Region-1-R-F 2 CTCAATGGTGGCAGTTCGCTG | Region-1-R-R2 TTCTTTCCCAAGACCAACACATGC | 398 | |
RpoB Las primer | RpoB-las-F AATTTTTCTGTTCCTCGCAGC | RpoB-las-R CAGCGAACTGCCACCATTGAG | 1,530 |
RpoB Las & Laf common primer pair | RpoB-las-laf-F TCAACTTGAAGAACATGTGAACTCTCTTTCGC | RpoB-las-laf-R CAGCGAACTGCCACCATTGAG | 1,508 |
Region-2 | |||
Region-2-L-F GCGGGCCTGTTGATAATCCTGC | Region-2-L-R CACATAACCAAGTCAACCCA | 1,931 | |
Region-2-R-F CAACAACCCTGACCTCCATC | Region-2-R-R CACAGTTTCATAGCCTCCCA | 1,041 | |
Region-3 | |||
Region-3-L-F GCAGCTGGAAGGGGATTCAC | Region-3-L-R GATAGCACCCTGATATTACACAA | 4,150 | |
Region-3-R-F GAGGAACCGTTGAGTATGGC | Region-3-R-R TTTCTACAGTCTACGATGCG | 1,124 |