Figure 3
From: SP1 enhances Zbtb7A gene expression via direct binding to GC box in HePG2 cells
Sp1 increased basal promoter activity could be abrogated by the GC boxes mutation. A, Sp1 enhances the core promoter activity of Zbtb7Agene. HepG-2 and 293 T cells were transient co-tranfected with either pLuc-1000 and pCDNA3.1, or Sp1 and pLuc-10000 or SpAm or SpBm. pGl4.10 was control B, RT-PCR analysis of Zbtb7AmRNA using the total RNA was isolated from 293 T cells tranfected Sp1.GAPDH was control. Data for A means for ± SD from three independent experiments, with each experiment carried out in triplicate.