Figure 3

RT-PCR analysis of Cu/ZnSOD expression in response to cold and UV stresses conditions. RNA was extracted from leaves of plants subjected to stress conditions. Plants cultivated in laboratory at 13°C (not acclimated) and at 4°C (acclimated) (Lane 1 and 2). Plants growing in the Antarctic without UV radiation and under ambient light (UV radiation) (Lane 3 and 4). Controls: genomic DNA of D. antarctica (300 ng) (Lane 5), RNA at 4°C (acclimated) (Lane 6) with primers specific to amplify psbA gene. DNA from E. coli::pGEM-T Easy-SOD clone (50 ng) (Lane 7) was used as positive control. Deionised water, negative control (Lane 8). MW: Molecular weight marker (Fermentas). Reverse transcription of 1 μg samples of total RNA was carried out, followed by conventional PCR amplification using gene-specific primers. Reaction products (20 μl) were analyzed by gel electrophoresis.