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Table 2 Rescue of the aplnra MO epiboly phenotype by co-injection of aplnra mRNA

From: Zebrafish aplnra functions in epiboly

Extent of epiboly at 8 hpf

MO3 alone

MO3 + mRNA

 

% (n)

% (n)

75% epiboly - bud

4.8 (7)

21 (30)

50-75% epiboly

49.0 (71)

52.4 (75)

30-50% epiboly

46.2 (67)

26.6 (38)

Total number injected (n)

(145)

(143)

  1. MO3 at 1 mM was injected into the embryos at the 1-cell stage. aplnra mRNA at 100 ng/μL was injected into the yolk cell immediately vegetal to the embryo at the 32-cell stage. A contingency χ2 test of the data across all the phenotypic classes gives p < 0.001.
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BMC Research Notes

ISSN: 1756-0500

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