Figure 1From: TILLING in the two-rowed barley cultivar 'Barke' reveals preferred sites of functional diversity in the gene HvHox1Mutation frequency in relation to EMS concentration. The relative SNP rates for EMS treatments between 20 and 50 mM were calculated as particular sub-population specific frequencies. Data are based on the mutation screening of eight gene fragments in 7,389 M2 lines. The white and black bars show the accumulated synonymous and non-synonymous (AA changes and truncation) mutations detected for each sub-population. The relationship between SNP frequencies and EMS dosages was calculated based on a non-parametric Spearman rank correlation (coefficient of rs = 0.94 with a significance at p ≤ 0.01).Back to article page