TILLING in the two-rowed barley cultivar 'Barke' reveals preferred sites of functional diversity in the gene HvHox1

Table 4 List of TILLING targets, size of amplicons, and the number and distribution of mutation types

Gene	Description	Amplicon	Amplicon size (bp)¹⁾	Mutation					Frequency (Mb)⁵⁾
				total	Intron	syn²⁾	non-syn³⁾	trun⁴⁾
HvCO1 ⁶⁾	CONSTANS-like gene	1	830	8	-	5	3	-	1/0.67
Mlo9 ⁷⁾	Modulator of powdery mildew resistance and cell death	1	900	2	-	-	1	1	1/0.75
		2	1476	3	1	2	-	-	1/0.87
HveIF4E ⁸⁾	eukaryotic translation initiation factor 4E	1	367	7	3	-	4	-	1/0.28
		2	321	4	2	-	2	-	1/0.41
HvDnaJ-like ⁹⁾	DnaJ-like chaperone	1	434	7	2	2	3	-	1/0.36
HvHox1 ¹⁰⁾	Homeodomain transcription factor controlling row-type morphology in barley	1	985	22	7	6	8	1	1/0.3
		2	362	9	2	5	2	-	1/0.2
HvCIGR2 ¹¹⁾	Barley homolog of rice 'Chitin-inducible gibberellin-responsive'	1	699	13	-	10	2	1	1/0.34
		2	702	6	-	2	4	-	1/0.63

¹⁾ 7,389 M₂ lines were screened for mutations in the listed genes, except for the Mlo9 gene that was analysed in 1,920 M₂ lines. ²⁾ syn = synonymous mutations that do not alter the AA sequence of the protein; ³⁾ non-syn = non-synonymous mutations that introduce a change of AA in the protein sequence; ⁴⁾ trun = truncation mutations that generate a premature stop codon or alter a splice junction, potentially resulting in a truncation of the protein; ⁵⁾ The mutation frequency for each amplicon is calculated as follows: (size of amplicon --100 bp × total number of screened samples)/total number of identified mutations; 100 bp were subtracted because of the diminished ability to detect mutations in the upper and lower 50 bp; ⁶⁾ [64]⁷⁾ [65, 66]⁸⁾ [56]⁹⁾ NCBI accession BQ470183 ¹⁰⁾ [15]¹¹⁾ NCBI accession EU914128 (bp position 69,100-70,440)

ISSN: 1756-0500