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Table 3 Thermocycler conditions for preselective PCR.

From: Validation of phylogenetic signals in amplified fragment length data: testing the utility and reliability in closely related taxa

Preselective Cycles

Step

Temperature

Duration

Number of Cycles

Initial Denaturation

94°C

120 sec

1

Denaturation

94°C

50 sec

 

Annealing

56°C

60 sec

2

Extension

72°C

60 sec

 

Denaturation

94°C

50 sec

 

Annealing

56°C

60 sec

25

Extension

72°C

120 sec

 

Final Extension

72°C

120 sec

1

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BMC Research Notes

ISSN: 1756-0500

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