Validation of phylogenetic signals in amplified fragment length data: testing the utility and reliability in closely related taxa

Table 4 Cocktails used for selective PCR in AFLP.

Selective Cocktail
Reagent	Quantity (μl)
5 pmol Labeled Selective Eco RI Primer	1.5
15 pmol Unlabeled Selective Mse I Primer	1.5
10 mM dNTP	3.0
Formamide	0.5
25 mM MgCl2	3.0
H²O	10.0
10× PCR Buffer	2.5
Taq DNA Polymerase	0.5
Total	22.5**
** Add 20 μl allows for pipetting error
Reaction cocktails for selective PCR amplifications of the ligated DNA

ISSN: 1756-0500