Skip to main content

Table 5 Thermocycler conditions for selective PCR.

From: Validation of phylogenetic signals in amplified fragment length data: testing the utility and reliability in closely related taxa

Selective Cycles

Step

Temperature

Duration

Number of Cycles

Initial Denaturation

94°C

120 sec

1

Denaturation

94°C

50 sec

 

Annealing

56°C

60 sec

2

Extension

72°C

60 sec

 

Denaturation

94°C

50 sec

 

Annealing

56°C

60 sec

20

Extension

72°C

120 sec

 

Final Extension

72°C

10 min

1

Back to article page

BMC Research Notes

ISSN: 1756-0500

Contact us