Characterization of some Brucella species from Zimbabwe by biochemical profiling and AMOS-PCR

BMC Research Notes

Table 2 Basic biochemical and metabolic profiles of field Brucella spp. from Zimbabwe.

*Brucella* isolate	Biochemical properties						Growth on TSA in the presence of dyes
reference no.	Cat	Oxi	^a Ure	Mot	Ind	MZN	T ₂₀	T ₄₀	BF ₂₀
B1	+	+	+	-	-	+	-	-	+
B4	+	+	+	-	-	+	-	-	+
B5	+	+	+	-	-	+	-	-	+
B6	+	+	+	-	-	+	-	-	+
B7	+	+	+	-	-	+	-	-	+
B8	+	+	+	-	-	+	-	-	+
B9	+	+	+	-	-	+	-	-	+
B10	+	+	+	-	-	+	+	+	+
B12	+	+	+	-	-	+	-	-	+
B14	+	+	+	-	-	+	-	-	+
B15	+	+	+	-	-	+	-	-	-
B16	+	+	+	-	-	+	-	-	+
B20	+	+	+	-	-	+	-	-	-
B21	+	+	+	-	-	+	-	-	+

Cat, Catalase; Oxi, Oxidase, Ure; Urea hydrolysis; Mot, Motility test (+ = motile, - = non-motile); Ind, Indole production; MZN, Modified Ziehl Neelsen stain; TSA, Tryptone Soya agar; T₂₀, 20 μl/ml thionin; T₄₀, 40 μl/ml thionin; BF₂₀, 20 μl/ml basic fuchsin; + = positive test result; - = negative test result.
^aUrea hydrolysis = All isolates positive within 2 hours of culture

ISSN: 1756-0500