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Table 1 Names, function, database ID and annealing temperature (Ta) of the tested primer sets

From: Validation of internal reference genes for quantitative real-time PCR in a non-model organism, the yellow-necked mouse, Apodemus flavicollis

Abbreviation

Gene

Function

Accession Number

Ta [°C]

Actb-1

actin, beta

cytoskeletal structural protein

2848#

60°C

Actb-2

actin, beta

involved in cell motility, structure and integrity

ensmusg00000029580+

55°C

Actg1

actin, gamma, cytoplasmic1

Cytoskeletal structural protein

ensmusg00000062825+

55°C

B2 m-1

beta-2 microglobulin

cytoskeletal protein involved in cell locomotion

3584#

60°C

B2 m-2

beta-2 microglobulin

cytoskeletal protein involved in cell locomotion

ensmusg00000060802+

55°C

Canx

calnexin

protein folding and quality control in the endoplasmic reticulum

ensmusg00000020368+

55°C

Gapdh

glyceraldehyde-3-phosphate dehydrogenase

carbohydrate metabolism

3244#

60°C

Hprt1

hypoxanthine guanine phosphoribosyl transferase 1

metabolic salvage of purines in mammals

50#

55°C

Pgk1

phosphoglycerate kinase 1

transferase enzyme in the glycolysis

ensmusg00000062070+

55°C

Sdha

succinate dehydrogenase complex, subunit A

tricarboxylic acid cycle

ensmusg00000021577+

55°C

Rpl13a

ribosomal protein L13A

member of ribosome protein

enst00000270634+

55°C

Rplp0

ribosomal protein, large, P0

member of ribosome protein

2861#

60°C

Rps18

ribosomal protein S18

member of ribosome protein

ensmusg00000008668+

55°C

Tuba1a

tubulin, alpha 1A

structural protein

1484#

58°C

Ubc

Ubiquitin C

protein degradation

ensmusg00000008348+

55°C

  1. The eight reference genes that performed well in A. flavicollis liver samples are marked in bold.
  2. # RTPrimerDB: http://medgen.ugent.be/rtprimerdb
  3. + Ensembl Project: http://www.ensembl.org/index.html; Primer of Gene Normalization Panel