Validation of internal reference genes for quantitative real-time PCR in a non-model organism, the yellow-necked mouse, Apodemus flavicollis

Table 2 Descriptive statistics of the tested reference genes

	Rps18	Sdha	Canx	Pgk1	Actg1	Ubc	Rpl13a	Actb
GenBank ID	GU188049	GU188053	GU188051	GU188052	GU188050	GU188054	GU188056	GU188055
AM _{amplification rate}	1.86	1.88	1.83	1.85	1.88	1.86	1.85	1.82
CV _{amplification rate}	0.06	0.05	0.05	0.05	0.06	0.05	0.05	0.05
GM _Ct-value	14.62	15.37	16.63	15.72	16.83	15.72	26.05	16.05
AM _Ct-value	14.64	15.42	16.68	15.78	16.88	15.79	26.10	16.16
CV _Ct-value	0.04	0.07	0.07	0.07	0.07	0.08	0.06	0.09
Minimum _Ct-value	13.86	13.90	14.65	13.42	14.94	12.87	24.30	13.64
Maximum _Ct-value	16.74	17.68	19.61	18.62	19.68	18.26	28.87	20.50
SD _Ct-value	0.65	1.09	1.09	1.10	1.11	1.26	1.45	1.49
SD-threshold	1.12	1.10	1.15	1.13	1.10	1.12	1.13	1.16
Minimum [x-fold]	-1.60	-2.52	-3.30	-4.10	-3.29	-5.90	-2.94	-4.24
Maximum [x-fold]	3.71	4.27	6.10	5.93	6.00	4.84	5.65	14.49
SD [± x-fold]	1.50	1.99	1.99	2.00	2.01	2.21	2.49	2.56

Arithmetic mean (AM), geometric mean (GM), coefficient of variance (CV) and standard deviation (SD) of the amplification rate E and the Ct-values for every potential reference gene. The genes are ordered by their SD_Ct-value. Genes that showed a SD_Ct-valuesmaller than the SD-threshold are considered to be suitable reference genes and marked in bold. The last three rows show the maximum and minimum values of the over- and under-expression of a gene in relation to its calculated geometric mean (displayed as x-fold ratio) as well as the standard deviation (calculated with BestKeeper).

ISSN: 1756-0500