A SNaPshot assay for the rapid and simple detection of four common hotspot codon mutations in the PIK3CA gene

BMC Research Notes

Table 1 SNaPshot probes for the detection of PIK3CA mutations.

Probe	Sequence (5'->3')	Size (bp)	Mutation	Concentration in probe mix (μM)
E542K	T₍₁₉₎TACACGAGATCCTCTCTCT	38	G->A	0.8
E545G	T₍₂₉₎TCCTCTCTCTGAAATCACTG	49	A->G	2.3
E545K	T₍₃₄₎ATCCTCTCTCTGAAATCACT	54	G->A	1.5
H1047R	T₍₄₆₎TGAAACAAATGAATGATGCAC	67	A->G	1.5

ISSN: 1756-0500