Table 1 Primer sequences used for chromosome and genome walking
From: A specific insertion of a solo-LTR characterizes the Y-chromosome of Bryonia dioica (Cucurbitaceae)
Primer sequences for chromosome walking on the Y-chromosome | |
|---|---|
GSP7 | 5'-TGCATTTCTACAAGCTATCACCGACC |
GSP8 | 5'-AGAGACCGTGGGACATGGTGTCACATACCTC |
GSP16 | 5'-GCCGTGCATAGGAGCAGACTC |
GSP17 | 5'-AGTGGGTGAGAGGTATGTGACACCATGTC |
GSP18 | 5'-ACGGTCTCTTCCATTAGGTCGGTGATAGC |
GSP20 | 5'-AAGGAAGAATTGCGCCAGCAC |
GSP21 | 5'-CAGCTGATCAAGAGTTACTCCCAGTCAACTG |
GSP22 | 5'-TCCGGATGCGGTGGCGCATTG |
GSP23 | 5'-AATGCGCCACCGCATCCGGAC |
GSP24 | 5'-TCAGGGACCATGACGGTTGC |
Primer sequences for genome walking to recover retrotransposon sequence | |
GSP15 | 5'-GGTCTCTTCCATTAGGTCGCACCGTGAG |
GSP17 | 5'-AGTGGGTGAGAGGTATGTGACACCATGTC |
GSP19 | 5'-ACTACCCTTCGGTAGATGTTGC |
GSP19-1 | 5'-TGCAATCAAGACGGGCATTGG |
GSP19-7 | 5'-CCTTAGAGCTCGTACATTCGGAC |
GSP19-8 | 5'-CCCAGCAGAACGGTGTATCAG |
GSP19-9 | 5'-TGGATATGGTTCGCTCTATGATGAGC |
GSP19-12 | 5'-ATGGTTCGCTCTATGATGAGC |
GSP19-14 | 5'-GATGGCGTAGAGGATCCACTG |
GSP19-15 | 5'-TGATGTGGATCGTGACCAGTGG |