Figure 4From: A general framework for optimization of probes for gene expression microarray and its application to the fungus Podospora anserinaImprovement of probe reproducibility in microarray v.3. The distributions of probe intensity CV are presented in a series of six boxes (interquartile range) and whiskers plots. Hybridizations were performed with the reference common cRNA pool on microarray v.2 (5 hybridizations, REF_v.2_M24h, REF_v.2_M48h, REF_v.2_M96h, REF_v.2_C24h, REF_v.2_C48h, each with 4 technical replicates per probe) and v.3 (REF_v.3, 12 technical replicates per probe). The cRNAs were labelled with Cy5. The median CV is 0.14, 0.10, 0.16, 0.10, 0.09 and 0.05 for REF_v.2_M24h, REF_v.2_M48h, REF_v.2_M96h, REF_v.2_C24h, REF_v.2_C48h and REF_v.3, respectively.Back to article page