Expression analysis of flavonoid biosynthesis genes during Arabidopsis thaliana silique and seed development with a primary focus on the proanthocyanidin biosynthetic pathway

Table 1 Definition of stages for A. thaliana seed and silique development used in this work.

stage	stages of embryogenesis	hours after flowering	stage length¹ [mm]	sample harvested for ATH1 GeneChip hybridization	AtGen-Express accession (ATGE) No.
0	mature embryosac	0	1.2-1.4	-	-
1	up to four cells	12-24	2.0-2.2	-	-
2	early globular to mid globular	24-48	2.4-2.8	-	-
3	mid globular to early heart	48-66	3.8-4.2	siliques containing seeds	ATGE_76
4	early heart to late heart	66-84	6.0-6.8	siliques containing seeds	ATGE_77
5	late heart to mid torpedo	84-90	8.0-9.2	siliques containing seeds	ATGE_78
6	mid torpedo to late torpedo	90-96	10.0-11.2	isolated seeds	ATGE_79
7	late torpedo to early walking-stick	96-108	11.6-12.2	isolated seeds	ATGE_81
8	walking-stick to early curled cotyledons	108-120	12.4- 13.0	isolated seeds	ATGE_82
9	curled cotyledons to early green cotyledons	120-144	12.8-13.6	isolated seeds	ATGE_83
10	green cotyledons	144-192	13.6-14.4	isolated seeds	ATGE_84

¹Minimal and maximal flower and silique length observed under our green house conditions.

ISSN: 1756-0500