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Figure 1 | BMC Research Notes

Figure 1

From: Allelic replacement of the streptococcal cysteine protease SpeB in a Δsrv mutant background restores biofilm formation

Figure 1

Construction of MGAS5005Δ srv Δ speB. (A) speB flanking sequences were cloned upstream and downstream of the chloramphenicol resistance cassette cat (Cmr) in pFW14. The resulting plasmid was transformed into MGAS5005Δsrv, and allelic replacement yielded MGAS5005Δsrv ΔspeB. (B) PCR of (I) MGAS5005, (II) MGAS5005Δsrv, (III) MGAS5005ΔspeB and (IV) MGAS5005Δsrv ΔspeB using primers srv internal FWD/REV (347 bp fragment) and internal speB FWD/REV (80 bp fragment) to verify deletion of the genes srv and speB within the MGAS5005 mutants. Ladder (L) is a 1 kB ladder.

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