A ranking index for quality assessment of forensic DNA profiles

BMC Research Notes

Table 3 Electropherogram data for the DNA profile in Figure 1B

Locus	Allele 1	Peak height (rfu)	Allele 2	Peak height (rfu)	TPH	MLB	SH	FI
D3S1358	14	671	17	706
vWA	15	714	17	710
D16S539	12	227	13	253
D2S1338	17	442
D8S1179	14	416	15	431	7284	0.96	2.19	1.59
D21S11	32.2	351	33.2	317
D18S51	16	198	19	190
D19S433	14	624
TH01	9	557
FGA	21	234	27	243

The DNA profile was obtained by analysing a swab of a spoon found in a honey jar, DNA concentration 0.09 ng/μl, using an alternative DNA polymerase and AmpFl STR SGM Plus primers. The STR markers D2S1338, D19S433 and TH01 are homozygous (one allele expected), the other markers are heterozygous (two alleles expected). Peak heights are given in relative fluorescence units (rfu). Total sum of Peak Heights (TPH), Mean Local Balance (MLB), Shannon entropy (SH) and the calculated forensic DNA profile index (FI) are presented.

ISSN: 1756-0500