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Figure 1 | BMC Research Notes

Figure 1

From: Comparative expression pattern of Matrix-Metalloproteinases in human glioblastoma cell-lines and primary cultures

Figure 1

Expression analysis of MMPs in glioblastoma cell-lines and primary cells by semiquantitative RT-PCR. Total RNA from the seven glioblastoma cell-lines SNB-19, GaMG, U251, U87, U373, U343 and U138 was used as template for semiquantitative RT-PCR analysis. Cells were passaged fifteen times and samples of total RNA were isolated from passage 0 (p0), passage 5 (p5) and passage 15 (p15). Primers were designed in flanking exons specific for each transcript (Table 2). The length of the cDNA amplicons in base pairs (bp) is shown on the right side of the figure. As positive control (+) genomic DNA from GaMG cells was used, except for MMP-10 and MMP-24 where cDNA of HBMEK cells and for GAPDH where cDNA from U87 cells served as positive control. The various cDNA concentrations were normalized to that of the housekeeping gene GAPDH, which was used as internal loading control. Shown is one representative experiment out of six.

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