Table 2 Primers and conditions used for semiquantitative RT-PCR screening
Gene | Forward primer | Reverse primer | Tm (°C) | Cycles | cDNA fragment size (bp) | Genomic DNA (bp) |
|---|---|---|---|---|---|---|
MMP-1 | 5'-AAGGCCAGTATGCACAGCTT-3' | 5'-TGCTTGACCCTCAGAGACCT-3' | 57 | 32 | 480 | 1.060 |
MMP-8 | 5'-TCTGCAAGGTTATCCCAAGG-3' | 5'- ACCTGGCTCCATGAATTGTC-3' | 57 | 32 | 154 | 852 |
MMP-9 | 5'-CCTGCCAGTTTCCATTCATC-3' | 5'-GCCATTCACGTCGTCCTTAT-3' | 58 | 32 | 455 | 957 |
MMP-10 | 5'-CCAGTCTGCTCTGCCTATCC-3' | 5'- CATCTCAGATCCCGAAGGAA-3' | 55 | 32 | 819 | 4.199 |
MMP-11 | 5'-GGGGATGTCCACTTCGACTA-3' | 5'-CAGTGGGTAGCGAAAGGTGT-3' | 50 | 32 | 165 | 308 |
MMP-13 | 5'-AACATCCAAAAACGCCAGAC-3' | 5'-GGAAGTTCTGGCCAAAATGA-3' | 57 | 32 | 166 | 1.117 |
MMP-17 | 5'-GGAGCTGTCTAAGGCCATCA-3' | 5'-CGACAGGTTCCTCTTGTTCC-3' | 56 | 32 | 190 | 474 |
MMP-19 | 5'-CAGCCTCGTTGTGGCCTAGA-3' | 5'-ACCAGCCTGCACCTCTTGGA-3' | 55 | 32 | 207 | 430 |
MMP-20 | 5'-CGACAATGCTGAGAAGTGGA-3' | 5'-ATCTTTGGGGAGGTGGAATC-3' | 57 | 32 | 169 | 975 |
MMP-21 | 5'-GACGACGACGAGCACTTCAC-3' | 5'-TTTCCTGTCTGACCAGTCCA-3' | 53 | 32 | 180 | 431 |
MMP-23 | 5'-TGGGACCACTTCAACCTCAC-3' | 5'-CGTGTTGTGAGTGCATCAGG-3' | 55 | 32 | 412 | 938 |
MMP-24 | 5'-GAACCTGTGGGCAAGACCTA-3' | 5'-TGACAACCAGAAACTGAGCG-3' | 52 | 32 | 214 | 2.650 |
MMP-26 | 5'-GATATGAAGCCATCCGCAGT-3' | 5'-GCTGGAAGGTTCTAGGGTCG-3' | 58 | 32 | 378 | 1.470 |
MMP-27 | 5'-TTGTTTCTTGTGGCTGCTCA-3' | 5'-GCTAAGCCAAAGGAACCCAC-3' | 53 | 32 | 194 | 376 |
MMP-28 | 5'-CACCTCCACTCGATTCAGCG-3' | 5'-AAAGCGTTTCTTACGCCTCA-3' | 57 | 32 | 208 | 376 |
GAPDH | 5'-GCAGGGGGGAGCCAAAAGGG-3' | 5'-TGCCAGCCCCAGCGTCAAAG-3' | 68 | 21 | 566 | 851 |