Figure 1

5 novel germline APC mutations identified by sequencing analysis. Results of sequencing the APC gene locus in DNA derived from the blood of Japanese (A)FAP patients. An asterisk indicates the location of the mutation. a. Results of sequencing analysis of the PCR product covering the c.446A > T and c.448A > T mutations. An amino acid sequence based on the mutated nucleotide sequence is shown below the electropherograms. b. Results of sequencing analysis of the subcloned PCR product covering the c.454_457insAGAA mutation. An amino acid sequence based on the mutated nucleotide sequence is shown below the electropherograms. c. Results of sequencing analysis of the subcloned PCR product covering the c.497insA mutation. An amino acid sequence based on the mutated nucleotide sequence is shown below the electropherograms. d. Results of sequencing analysis of the PCR product covering the c.1958G > C mutation. The boundary between exon 14 and intron 14 is indicated by a vertical line.