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Table 2 Fluorescent Genotyping Assays.

From: Association analysis of PRNP gene region with chronic wasting disease in Rocky Mountain elk

Sequence variant

Reagent Role

Oligonucleotide

g.655C > T2

Primer

GCCTGGACTCCACCTTAGG

 

Primer

CGATGAGTAACCTGGATACGATCAA

 

Probe4

TTCTGTTGTCGTTTCCT

 

Probe5

TCTGTTGTCATTTCCT

g.1131C > G2

Primer

GCTACAACAGACTGACTGGATAGAA

 

Primer

GGAATGAAGATGATTCAGTAATGGAAATGG

 

Probe4

CCAGTTAGAAATCAGTATTA

 

Probe5

CCAGTTAGAAATGAGTATTA

g.18390A > T2

Primer

GCAGCTGGAGCAGTGGTA

 

Primer

CAAAATGTATAAGAGGCCTGCTCATG

 

Probe4

TTCCCAGCATGTAGCC

 

Probe5

CCCAGCAAGTAGCC

g.1456C > G2

Primer

GGAGCCTCTTGCCCATAACAA

 

Primer

AGAAAACAGGTGTTAGAGGGATCATATACA

 

Probe4

ATTTATGCTGGGTCAATG

 

Probe5

TTATGCTGCGTCAATG

g.17361G > A2

Primer

TCTGTGCCAGGCTCTGC

 

Primer

AGGGAATGAGTCTCTTTTGTTTGCT

 

Probe4

CCTGGCCCACTAAGA

 

Probe5

CTGGCCCGCTAAGA

g.1104C > T3

Primer

CCATGTGGAGGCCAGAAATCC

 

Primer

GATGCCAGAAGAGTTTACAGAGACA

 

Probe4

CTGGAGGTGGGAAAC

 

Probe5

CTGGAGGTAGGAAAC

g.986A > G1

Primer

ATCCCCTACCTATGACTTTCAGACA

 

Primer

TCCCCATAACCCGGAATCCT

 

Probe4

ATCATAGGCTACTACCTCATT

 

Probe5

TAGGCTACTGCCTCATT

g.371T > C1

Primer

GGACTGTCTAGATGAGCCAAACAT

 

Primer

AGAGTCCCAGTTGCAGCTTAG

 

Probe4

AGGATGACCGAAATAT

 

Probe5

AGGATGACCAAAATAT

g.18308G > A2

Primer

CACATGGTGGTGGAGGCT

 

Primer

GCTCCTGCCACATGCTTCA

 

Probe4

TCAGTGGAACAAACCCAGT

 

Probe5

AGTGGAACAAGCCCAGT

  1. 1Nucleotide positions are based on GenBank:FJ590753.
  2. 2Nucleotide positions are based on GenBank:FJ590751.
  3. 3Nucleotide positions are based on GenBank:FJ590752.
  4. 4Taqman probe labelled with VIC dye. 5Taqman probe labelled with FAM dye.