Figure 2
Co-localization of A 1 R and P2Y 2 R. A-C. Confocal images of double immunostained Myc-P2Y2R (A; green), HA-A1R (B; red), and their merge (C; yellow) in co-transfected HEK293T cells. The co-localization of HA-A1R and Myc-P2Y2R is evident at the cell surface membrane (small arrow). D-L. Confocal images of double immunofluorescence staining in several rat brain regions. P2Y2R (D, G, J; red) and A1R (E, H, K; green) immunoreactivities were detected in Purkinje cells (D-F), cerebellar nuclei (G-I), and hippocampal CA3 pyramidal cells (J-L). Co-localizations of A1R and P2Y2R (F, I, L; yellow) were detected in the soma (large arrows) of all tissues, in dendrites of the Purkinje cells, and in neurons of the cerebellar nuclei (arrowheads). Yellow bar indicates 500 μm (A-C) and white bar indicates 100 μm (D-L). Mol: cerebellar molecular layer, Gr: cerebellar granule cell layer. Fluorescent images were collected via confocal laser scanning microscopy (Zeiss LSM410, Carl Zeiss, Oberkochen, Germany) each 10-μm optical slice consisted of a stack of 20 0.5-μm thick sections. Serial optical sections were recorded using an air objective lens of (40×, numerical aperture; 0.6).