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Figure 4 | BMC Research Notes

Figure 4

From: Immunogold electron microscopic evidence of in situ formation of homo- and heteromeric purinergic adenosine A1 and P2Y2 receptors in rat brain

Figure 4

Immunogold electron microscopy of A 1 R and P2Y 2 R visualized using nanogold particles in transfected HEK293T cells (A-D) and rat brain (E-G). A: Localization of HA-A1R (large particles) detected with anti-HA in HA-A1R-transfected HEK293T cells. B: Localization of Myc-P2Y2R (small particles) detected with anti-Myc in Myc-P2Y2R-transfected HEK293T cells. C: Anti-HA and anti-Myc immuno-localization of HA-A1R and Myc-P2Y2R in co-transfected HEK293T cells. D: HA-A1R-transfected HEK293T cells incubated with both anti-HA and anti-Myc. E-G: Localization of A1R and P2Y2R in cortical pyramidal cells (E), Purkinje cells (F), and hippocampal pyramidal cells (G) detected with both anti-A1R and anti-P2Y2R. Arrows indicate two adjacent receptors on the cell membrane. Bars represent 100 nm. CM, cell membrane; CP, cytoplasm.

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BMC Research Notes

ISSN: 1756-0500

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