Figure 1

Structure of the APP promoter and the 5' UTR. (A) Schematic representation of the APP promoter with boxes indicating locations of regulatory regions and the DAPB domain discussed in the text. (B) The sequence of the 5'-APP-UTR from position +1 to +147. The translational start site (ATG) would follow position +147 in the native APP gene. Also shown are deletions at position +10, +30, +50, and +70 (arrows) and pertinent nucleotide positions as discussed in the text. Brackets show relevant restriction sites, of which SalI is part of the plasmid polylinker region. The sequence defining DNase I protected domain DAPB (solid lines) and the sequence recognized by the binding factor (dotted lines) are indicated by boxes. (C) Schematic representation of internal deletions D [10]-D [70] within the APP [147] fragment. Deleted sequences are indicated by dotted lines.