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Figure 2 | BMC Research Notes

Figure 2

From: A nuclear factor-binding domain in the 5'-untranslated region of the amyloid precursor protein promoter: Implications for the regulation of gene expression

Figure 2

Expression from the APP promoter with deletions within the 5'- APP -UTR. (A) Expression from the full-length APP promoter and internal 5'-APP-UTR deletions by transient transfection in HeLa cells. The results are presented as CAT activities relative to the wild type APP [147] construct, which was assigned the value of 100% (column 1). Columns 2-5 show internal deletions D [70]-D [10] within APP [147] and column 6 shows the APP [104] construct that terminates at position +104. The results represent the average values of at least ten separate transfections with standard deviations (error bars). (B) Run-off in vitro transcription from promoter construct APP [104] (lane 1), APP [147] (lane 2) and sequential deletions D [70]-D [10] (lanes 3-6). The bracket delineates fragments transcribed from the APP promoter. Arrows indicate the two transcripts originating from the β-actin promoter. (C) Quantitation of transcription reactions illustrated in B. The APP transcripts were normalized to identical β-actin transcripts and the full-length (FL) APP [147] construct was assigned the value of 100% (column 2). Results represent the average of four independent experiments with standard deviations (error bars).

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