Figure 4
CTGF expression in PGE 2 -stimulated chondrocytes: Western blot. Top: Chondrocyte samples (n = 5) were either stimulated by PGE2 (10 nM) or not for 24 h, with or without pretreatment with an EP-specific antagonist (AH: AH6809, 10 ng/ml; GW: GW627368X, 5 μM), for 1 h in vitro. Bottom: The relative value of band intensity (CTGF/GAPDH) was calculated using an image analyzer.