Figure 4

Effect of Sodium halides on pH dependent changes in functional activity and structural features of EhPSAT. (A) Inhibition of functional activity in presence of 200 mM concentration of various Sodium halides. The activity of EhPSAT in absence of salt (native protein) at pH 8.5 was taken as 100%. (B) pH dependent enzymatic activity profile of EhPSAT in presence of 200 mM concentration of NaCl (rectangles) and NaF (circles), respectively. For samples in presence of NaCl and NaF the highest value observed was taken as 100%. Fluorescence emission spectra of EhPSAT in presence of 200 mM NaCl (Panel C) and NaF (Panel D) at different pH. The different curves in both the panel represent protein samples at pH 6 (solid line), 7 (dashed line), 8 (dotted line) and 9 (dash dotted line). SEC profile at various pH in presence of NaCl (panel E) and NaF (panel F). The curves 1-4 represent SEC profile in presence of 200 mM concentration of salts at pH 6, 7 and 8, and 9 respectively.