From: An enhanced method for sequence walking and paralog mining: TOPO® Vector-Ligation PCR
Primer Name | Primer Sequence | Tm (°C) * |
---|---|---|
Vector Primers | ||
M13F | 5'-GTAAAACGACGGCCAG-3' | 50.7 |
M13R | 5'-CAGGAAACAGCTATGAC-3' | 47.0 |
T3 | 5'-ATTAACCCTCACTAAAGGGA-3' | 50.3 |
T7 | 5'-TAATACGACTCACTATAGGG-3' | 47.5 |
Gene Primers | ||
14321 5' F1 | 5'-AGGGTTAGGTTTAAGGTTGAG-3' | 51.8 |
14321 5' F2 | 5'-GTGAGGGTACGAAAGTAGG-3' | 51.9 |
14321 3' F1 | 5'-TCGCAAGTTGAACTATGTATCC-3' | 52.6 |
14321 3' F2 | 5'-CAGTTTGTTCAGGACTGAGT-3' | 52.2 |