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Table 1 Primer Information.

From: An enhanced method for sequence walking and paralog mining: TOPO® Vector-Ligation PCR

Primer Name

Primer Sequence

Tm (°C) *

Vector Primers

  

M13F

5'-GTAAAACGACGGCCAG-3'

50.7

M13R

5'-CAGGAAACAGCTATGAC-3'

47.0

T3

5'-ATTAACCCTCACTAAAGGGA-3'

50.3

T7

5'-TAATACGACTCACTATAGGG-3'

47.5

Gene Primers

  

14321 5' F1

5'-AGGGTTAGGTTTAAGGTTGAG-3'

51.8

14321 5' F2

5'-GTGAGGGTACGAAAGTAGG-3'

51.9

14321 3' F1

5'-TCGCAAGTTGAACTATGTATCC-3'

52.6

14321 3' F2

5'-CAGTTTGTTCAGGACTGAGT-3'

52.2

  1. Vector primer sequences are standard, and are provided here for convenience. Gene primers were designed by us.
  2. *Melting temperature values were determined by the Integrated DNA Technologies OligoAnalyzer application [7], and may not be the same as those published elsewhere for M13_ and T_ primers.