Figure 4

T. brucei BSF parasites cultured under low glucose conditions possess reduced ATP levels. A. T. brucei BSF parasites were either cultured in RPMI-1640 media containing 0 mM or 25 mM D-glucose or 10 mM 2-deoxy-D-glucose, all supplemented with dialyzed (glucose-free) 10% FBS. A reduction in ATP was observed in parasites cultured in either 0 mM D-glucose or 10 mM 2-deoxy-D-glucose. Data are normalized to 100% for 25 mM D-glucose and are the mean of three independent assays. Error bars show the standard deviation. B. T. brucei BSF parasites were cultured in RPMI-1640 media containing 5 mM D-glucose and dialyzed (glucose-free) 10% FBS for twelve hours and ATP levels measured at intervals. Data are the mean of three independent determinations, and error bars show the standard deviation.