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Table 1 Multiplex PCR reverse cross blot hybridization results

From: Detection and identification of mycobacteria in sputum from suspected tuberculosis patients

PCR Probe Mycobacterium Species   Cultures
    (+)ve (-)ve
pMyc5a Mycobacterium spp 5'-GGGCCCATCCCACACCGC-3' 117 162
pAvi7 M. avium 5'CCAGAAGACATGCGTCTTGAG-3' 3 5
pInt5 M. intracellulare 5'-CACCTAAAGACATGCGCCTAA-3' 1 1
pInt7 M. intracellulare 5'-CACCAAAAGACATGCGTCTAA-3' 1 1
pKan7 M. kansasii 5'CAAGGCATGCGCCAAGTGGT-3' 1 1
pXen1 M. xenopi 5'-ACCACCCCACATGCGGAGAA-3' 0 0
pFor1 M. fortuitum 5'-ACCACACACCATGAAGCGCG-3' 1 1
pChe3 M. chelonae 5'-CCACTCACCATGAAGTGTGTG-3' 2 3
pGen1 M. genavense 5'-CCACAAAACATGCGTTCCGTG-3' 0 1
pGor5 M. gordonae 5'-TGTGTCCTGTGGTCCTATTCG-3 0 0
pMar2 M. marinum 5'-CGGGATTCATGTCCTGTGGT-3' 0 0
Pt3 M. tuberculosis complex 5'-GAACGGCTGATGACCAAACT-3' 117 162
pSme3 M. smegmatis 5'-CATGCGACCAGCAGGGTGTA-3' 1 1
pTub1 M. tuberculosis complex 5'-AACACAAGACATGCATCCCG-3' 108 149