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Table 3 Comparison of conventional multiplex PCR and PCR reverse cross blot hybridization assay

From: Detection and identification of mycobacteria in sputum from suspected tuberculosis patients

 

Conventional multiplex PCR positive/PCR Cross Blot Hybridization positive

 

Positive Culture (n = 136)

Negative culture (n = 251)

M. Tuberculosis

108/108

149/149

M. avium

3/3

5/5

M. intracellulare

0/1

0/1

M. kansasii

0/1

0/1

M. fortuitum

0/1

0/1

M. chelonae

0/2

0/3

M. genavense

0/0

0/1

M. smegmatis

0/1

0/1

No amplification

25/19

97/89