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Figure 1 | BMC Research Notes

Figure 1

From: Regulation of PCNA polyubiquitination in human cells

Figure 1

PCNA ubiquitination in normal skin fibroblasts. (A) GM038 cells were either left untreated or UV irradiated with 30 J/m2 and lysed 3 h post-treatment followed by immunoblotting for PCNA. (B) GM038 cells were irradiated with 30 J/m2 UV and lysed in boiling SDS, diluted in lysis buffer and subjected to immunoprecipitation with a PCNA antibody and detected with an anti-ubiquitin antibody. (C) GM038 cells were transfected with 100 nM of either control siRNA or siRNA RAD18. 72 hours post-transfection cells were treated as in figure 1A.

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