Figure 1From: Regulation of PCNA polyubiquitination in human cellsPCNA ubiquitination in normal skin fibroblasts. (A) GM038 cells were either left untreated or UV irradiated with 30 J/m2 and lysed 3 h post-treatment followed by immunoblotting for PCNA. (B) GM038 cells were irradiated with 30 J/m2 UV and lysed in boiling SDS, diluted in lysis buffer and subjected to immunoprecipitation with a PCNA antibody and detected with an anti-ubiquitin antibody. (C) GM038 cells were transfected with 100 nM of either control siRNA or siRNA RAD18. 72 hours post-transfection cells were treated as in figure 1A.Back to article page