A real-time PCR assay with improved specificity for detection and discrimination of all clinically relevant Bordetella species by the presence and distribution of three Insertion Sequence elements

Table 2 Results of PCRs for detection of IS 481, IS 1001, and IS 1002, and the interpretation for identification of clinically relevant Bordetella species

*Bordetella* subspecies*	IS 481	IS 1001	IS 1002	identification
pertussis	+	-	+	B. pertussis
parapertussis	-	+	+	B. parapertussis
holmesii	+	-	-	B. holmesii/B. bronchiseptica
bronchiseptica	-	+	-	B. bronchiseptica
petrii	-	-	-	identification not possible
hinzii	-	-	-	identification not possible

*Strains were kindly provided by Dr. Frits Mooi and Kees Heuvelman, Laboratory for Vaccine Preventable Diseases, National Institute of Public Health and the Environment, Bilthoven, The Netherlands

ISSN: 1756-0500