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Figure 2 | BMC Research Notes

Figure 2

From: SNPmplexViewer--toward a cost-effective traceability system

Figure 2

Fragment analysis of a 25-plex SNaPshot reaction without size standards. SNaPshot reaction products of the 25-plex cattle traceability system [2] were separated by capillary electrophoresis on an ABI310 sequencer and analysed with GeneMapper software. The genotypes of each SNP and the SNP bins are indicated in boxes below and above the trace, respectively. Genotypes are coloured in green, black, blue and red for A, C, G and T nucleotides, respectively. Bins are shaded following the same colour scheme except for the C bins, which are yellow. (A) Electropherogram from conventional analysis of an individual using GeneScanâ„¢ -120 LIZâ„¢ size standard (orange peaks). (B) Electropherogram from the analysis of the same individual with no size standards using a modified trace file, which was obtained as SNPmplexViewer output.

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