Figure 4
From: The importance of RT-qPCR primer design for the detection of siRNA-mediated mRNA silencing
Apparent knockdown of mRNA in mouse tumours is primer pair-specific. Hepa 1-6 tumours (n = 5) were injected with the indicated siRNAs and ApoB (A, B) or Rrm1 (C, D) mRNA levels measured by real-time RT-qPCR using primers flanking the siRNA target site (B, D) or outside this region (A,C). mmApoB is an inactive mismatched siRNA control; 81C is a control with no known mRNA target, pbs - PBS-injected tumours.