Figure 2From: Assessment of replicate bias in 454 pyrosequencing and a multi-purpose read-filtering toolDuplication profile before pyrocleaning and after. Simulated dataset were produced using the E coli K12 genome. Sequences of 500 bp were picked randomly along the genome using both strands. The number of simulated sequences (Sim run1/Sim run2) equals the number of sequences produced in the experimental runs (454 run1/454 run2).Back to article page