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Figure 1 | BMC Research Notes

Figure 1

From: Quality of tumor lysates used for pulsing dendritic cells is influenced by the method used to harvest adherent tumor cells

Figure 1

Tumor lysates from trypsinized tumor cells do not induce maturation of DCs. Adherent tumor cells were harvested by using 0.25% trypsin to detach the cells, washed and lysed in PBS by repeated freezing and thawing. Human monocyte-derived immature DCs were stimulated for 3 days with supernatants of indicated concentrations of lysed tumor cells. 12 individual experiments with lysates from 3 different cell lines (HCT-116, CACO-2, COLO-678) were performed and results with lysates from HCT-116 cells are shown as representative example.

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