Figure 1From: Quality of tumor lysates used for pulsing dendritic cells is influenced by the method used to harvest adherent tumor cellsTumor lysates from trypsinized tumor cells do not induce maturation of DCs. Adherent tumor cells were harvested by using 0.25% trypsin to detach the cells, washed and lysed in PBS by repeated freezing and thawing. Human monocyte-derived immature DCs were stimulated for 3 days with supernatants of indicated concentrations of lysed tumor cells. 12 individual experiments with lysates from 3 different cell lines (HCT-116, CACO-2, COLO-678) were performed and results with lysates from HCT-116 cells are shown as representative example.Back to article page