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Figure 3 | BMC Research Notes

Figure 3

From: Quality of tumor lysates used for pulsing dendritic cells is influenced by the method used to harvest adherent tumor cells

Figure 3

Trypsin abolishes maturation inducing effect of tumor lysate. Lysate from HCT-116 colorectal tumor cells which have been harvested by using 0.02% EDTA was were divided, one part was exposed to trypsin (0.25%) and the other part was sham treated with PBS for 5 min. In order to inactivate trypsin, 100% human AB serum (2× higher volume) was added to both arms after 5 minutes. DCs were stimulated for 3 days with trypsin-exposed or sham-treated tumor lysate and examined for expression of surface maturation markers CD40 and CD86.

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BMC Research Notes

ISSN: 1756-0500

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