Figure 5
An application of post-PCR labeling with BStag for SNP markers. Two SSR markers listed in Table 3 demonstrated successful labeling of each allele with different colors using BStag. A marker LLL0729 demonstrated segregation of C/G alleles for two different citrus hybrids (C/G: a hybrid of C. kunip × C. kinokuni; C/C: a hybrid of 'Lee' × C. kinokuni) labeled with NED (allele C) and PET (allele G). Another marker MAPGR75R also demonstrated segregation of A/T alleles for three citrus accessions: A/A for 'Hayasaki' (C. grandis 'Mato' × C. grandis 'Hirado'), T/T for a hybrid of C. kunip × C. kinokuni, and A/T for Satsuma mandarin.